The development of vaccines against SARS corona virus in mice and SCID-PBL/hu mice
Identifieur interne : 001786 ( Main/Exploration ); précédent : 001785; suivant : 001787The development of vaccines against SARS corona virus in mice and SCID-PBL/hu mice
Auteurs : Masaji Okada [Japon] ; Yuji Takemoto [Japon] ; Yoshinobu Okuno [Japon] ; Satomi Hashimoto [Japon] ; Shigeto Yoshida [Japon] ; Yukari Fukunaga [Japon] ; Takao Tanaka [Japon] ; Yoko Kita [Japon] ; Sachiko Kuwayama [Japon] ; Yumiko Muraki [Japon] ; Noriko Kanamaru [Japon] ; Hiroko Takai [Japon] ; Chika Okada [Japon] ; Yayoi Sakaguchi [Japon] ; Izumi Furukawa [Japon] ; Kyoko Yamada [Japon] ; Makoto Matsumoto [Japon] ; Tetsuo Kase [Japon] ; Daphne E. Demello [États-Unis] ; J. S. M. Peiris [Hong Kong] ; Pei-Jer Chen [Taïwan] ; Naoki Yamamoto [Japon] ; Yoshiyuki Yoshinaka [Japon] ; Tatsuji Nomura [Japon] ; Isao Ishida [Japon] ; Shigeru Morikawa [Japon] ; Masato Tashiro [Japon] ; Mitsunori Sakatani [Japon]Source :
- Vaccine [ 0264-410X ] ; 2005.
Descripteurs français
- KwdFr :
- Activation des lymphocytes, Animaux, Anticorps antiviraux (sang), Antigènes viraux (génétique), Femelle, Humains, Lymphocytes T cytotoxiques (immunologie), Protéines virales (génétique), Protéines virales (immunologie), Souris, Souris SCID, Souris de lignée BALB C, Souris de lignée C57BL, Souris de lignée NOD, Souris knockout, Techniques in vitro, Tests de neutralisation, Transfusion de lymphocytes, Transplantation hétérologue, Vaccins antiviraux (génétique), Vaccins antiviraux (isolement et purification), Vaccins à ADN (génétique), Vaccins à ADN (pharmacologie), Virus du SRAS (génétique), Virus du SRAS (immunologie).
- MESH :
- génétique : Antigènes viraux, Protéines virales, Vaccins antiviraux, Vaccins à ADN, Virus du SRAS.
- immunologie : Lymphocytes T cytotoxiques, Protéines virales, Virus du SRAS.
- isolement et purification : Vaccins antiviraux.
- pharmacologie : Vaccins à ADN.
- sang : Anticorps antiviraux.
- Pascal (Inist)
- Activation des lymphocytes, Animaux, Femelle, Humains, Souris, Souris SCID, Souris de lignée BALB C, Souris de lignée C57BL, Souris de lignée NOD, Souris knockout, Techniques in vitro, Tests de neutralisation, Transfusion de lymphocytes, Transplantation hétérologue, Virus syndrome respiratoire aigu sévère, Souris, Homme, Vaccin génétique, Grave, Lymphocyte T, Cytotoxicité, Immunodéficit mixte, Syndrome respiratoire aigu sévère, Mutation severe combined immunodeficiency (scid).
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (blood), Antigens, Viral (genetics), Combined immune deficiency, Cytotoxicity, Female, Genetic vaccine, Human, Humans, In Vitro Techniques, Lymphocyte Activation, Lymphocyte Transfusion, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Inbred NOD, Mice, Knockout, Mice, SCID, Mouse, Neutralization Tests, SARS Virus (genetics), SARS Virus (immunology), Severe, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus, T-Lymphocyte, T-Lymphocytes, Cytotoxic (immunology), Transplantation, Heterologous, Vaccines, DNA (genetics), Vaccines, DNA (pharmacology), Viral Proteins (genetics), Viral Proteins (immunology), Viral Vaccines (genetics), Viral Vaccines (isolation & purification).
- MESH :
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Antigens, Viral, Vaccines, DNA, Viral Proteins, Viral Vaccines.
- genetics : SARS Virus.
- immunology : SARS Virus, T-Lymphocytes, Cytotoxic, Viral Proteins.
- chemical , isolation & purification : Viral Vaccines.
- chemical , pharmacology : Vaccines, DNA.
- Animals, Female, Humans, In Vitro Techniques, Lymphocyte Activation, Lymphocyte Transfusion, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Inbred NOD, Mice, Knockout, Mice, SCID, Neutralization Tests, Transplantation, Heterologous.
Abstract
We have investigated to develop novel vaccines against SARS CoV using cDNA constructs encoding the structural antigen; spike protein (S), membrane protein (M), envelope protein (E), or nucleocapsid (N) protein, derived from SARS CoV. Mice vaccinated with SARS-N or -M DNA using pcDNA 3.1(+) plasmid vector showed T cell immune responses (CTL induction and proliferation) against N or M protein, respectively. CTL responses were also detected to SARS DNA-transfected type II alveolar epithelial cells (T7 cell clone), which are thought to be initial target cells for SARS virus infection in human. To determine whether these DNA vaccines could induce T cell immune responses in humans as well as in mice, SCID-PBL/hu mice was immunized with these DNA vaccines. As expected, virus-specific CTL responses and T cell proliferation were induced from human T cells. SARS-N and SARS-M DNA vaccines and SCID-PBL/hu mouse model will be important in the development of protective vaccines.
Affiliations:
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Higashinari-ku, Osaka 537-0025</wicri:noRegion></affiliation></author><author><name sortKey="Hashimoto, Satomi" sort="Hashimoto, Satomi" uniqKey="Hashimoto S" first="Satomi" last="Hashimoto">Satomi Hashimoto</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Yoshida, Shigeto" sort="Yoshida, Shigeto" uniqKey="Yoshida S" first="Shigeto" last="Yoshida">Shigeto Yoshida</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Infection and Immunity, Jichi Medical School, 3311-1 Yakushiji</s1><s2>Minamikawachi-machi, Tochigi 329-0498</s2><s3>JPN</s3><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Minamikawachi-machi, Tochigi 329-0498</wicri:noRegion></affiliation></author><author><name sortKey="Fukunaga, Yukari" sort="Fukunaga, Yukari" uniqKey="Fukunaga Y" first="Yukari" last="Fukunaga">Yukari Fukunaga</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Tanaka, Takao" sort="Tanaka, Takao" uniqKey="Tanaka T" first="Takao" last="Tanaka">Takao Tanaka</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Kita, Yoko" sort="Kita, Yoko" uniqKey="Kita Y" first="Yoko" last="Kita">Yoko Kita</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Kuwayama, Sachiko" sort="Kuwayama, Sachiko" uniqKey="Kuwayama S" first="Sachiko" last="Kuwayama">Sachiko Kuwayama</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Muraki, Yumiko" sort="Muraki, Yumiko" uniqKey="Muraki Y" first="Yumiko" last="Muraki">Yumiko Muraki</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Kanamaru, Noriko" sort="Kanamaru, Noriko" uniqKey="Kanamaru N" first="Noriko" last="Kanamaru">Noriko Kanamaru</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Takai, Hiroko" sort="Takai, Hiroko" uniqKey="Takai H" first="Hiroko" last="Takai">Hiroko Takai</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Okada, Chika" sort="Okada, Chika" uniqKey="Okada C" first="Chika" last="Okada">Chika Okada</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Sakaguchi, Yayoi" sort="Sakaguchi, Yayoi" uniqKey="Sakaguchi Y" first="Yayoi" last="Sakaguchi">Yayoi Sakaguchi</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Furukawa, Izumi" sort="Furukawa, Izumi" uniqKey="Furukawa I" first="Izumi" last="Furukawa">Izumi Furukawa</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Yamada, Kyoko" sort="Yamada, Kyoko" uniqKey="Yamada K" first="Kyoko" last="Yamada">Kyoko Yamada</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Matsumoto, Makoto" sort="Matsumoto, Makoto" uniqKey="Matsumoto M" first="Makoto" last="Matsumoto">Makoto Matsumoto</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Microbiological Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10, Kagasuno</s1><s2>Kawauchi-cho, Tokushima 771-019</s2><s3>JPN</s3><sZ>17 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Kawauchi-cho, Tokushima 771-019</wicri:noRegion></affiliation></author><author><name sortKey="Kase, Tetsuo" sort="Kase, Tetsuo" uniqKey="Kase T" first="Tetsuo" last="Kase">Tetsuo Kase</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Infectious Diseases, Osaka Prefectural Institute of Public Health</s1><s2>3-69 Nakamichi 1-chome Higashinari-ku, Osaka 537-0025</s2><s3>JPN</s3><sZ>3 aut.</sZ><sZ>18 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>3-69 Nakamichi 1-chome Higashinari-ku, Osaka 537-0025</wicri:noRegion></affiliation></author><author><name sortKey="Demello, Daphne E" sort="Demello, Daphne E" uniqKey="Demello D" first="Daphne E." last="Demello">Daphne E. Demello</name><affiliation wicri:level="2"><inist:fA14 i1="05"><s1>Department of Pathology Cardinal Glennon Children's Hospital, St. Louis University Health Science Center, 1465 South Grand Blvd</s1><s2>St. Louis, MO 63104</s2><s3>USA</s3><sZ>19 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Missouri (État)</region></placeName></affiliation></author><author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J. S. M." last="Peiris">J. S. M. Peiris</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Microbiology, The University of Hong Kong, Pokfulam Road</s1><s3>HKG</s3><sZ>20 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, The University of Hong Kong, Pokfulam Road</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Pei Jer" sort="Chen, Pei Jer" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Hepatitis Research Center, National Taiwan University College of Medicine, Room 328, 3F, No. 1, Sec. 1, Ren-ai Rd</s1><s2>Jhongjheng District 100, Taipei</s2><s3>TWN</s3><sZ>21 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Jhongjheng District 100, Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Tokyo Medical and Dental University, 1-5-45 Yushima</s1><s2>Bunkyo-ku, Tokyo 113-8549</s2><s3>JPN</s3><sZ>22 aut.</sZ><sZ>23 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Yoshinaka, Yoshiyuki" sort="Yoshinaka, Yoshiyuki" uniqKey="Yoshinaka Y" first="Yoshiyuki" last="Yoshinaka">Yoshiyuki Yoshinaka</name><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Tokyo Medical and Dental University, 1-5-45 Yushima</s1><s2>Bunkyo-ku, Tokyo 113-8549</s2><s3>JPN</s3><sZ>22 aut.</sZ><sZ>23 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Nomura, Tatsuji" sort="Nomura, Tatsuji" uniqKey="Nomura T" first="Tatsuji" last="Nomura">Tatsuji Nomura</name><affiliation wicri:level="1"><inist:fA14 i1="09"><s1>Central Institute for Experimental Animals, 1430 Nogawa, Miyamae</s1><s2>Kawasaki, Kanagawa 216-0001</s2><s3>JPN</s3><sZ>24 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Kawasaki, Kanagawa 216-0001</wicri:noRegion></affiliation></author><author><name sortKey="Ishida, Isao" sort="Ishida, Isao" uniqKey="Ishida I" first="Isao" last="Ishida">Isao Ishida</name><affiliation wicri:level="1"><inist:fA14 i1="10"><s1>Pharmaceutical Division, Kirin Brewery Co., 6-26-1 Jingumae</s1><s2>Shibuya, Tokyo 150-8011</s2><s3>JPN</s3><sZ>25 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name><affiliation wicri:level="1"><inist:fA14 i1="11"><s1>National Institute of Infectious Diseases, 1-23-1 Toyama</s1><s2>Shinjuku-ku, Tokyo 162-8640</s2><s3>JPN</s3><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name><affiliation wicri:level="1"><inist:fA14 i1="11"><s1>National Institute of Infectious Diseases, 1-23-1 Toyama</s1><s2>Shinjuku-ku, Tokyo 162-8640</s2><s3>JPN</s3><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Sakatani, Mitsunori" sort="Sakatani, Mitsunori" uniqKey="Sakatani M" first="Mitsunori" last="Sakatani">Mitsunori Sakatani</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">06-0198137</idno><date when="2005">2005</date><idno type="stanalyst">PASCAL 06-0198137 INIST</idno><idno type="RBID">Pascal:06-0198137</idno><idno type="wicri:Area/PascalFrancis/Corpus">000044</idno><idno type="wicri:Area/PascalFrancis/Curation">000015</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000044</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000044</idno><idno 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type="wicri:Area/Main/Exploration">001786</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">The development of vaccines against SARS corona virus in mice and SCID-PBL/hu mice</title><author><name sortKey="Okada, Masaji" sort="Okada, Masaji" uniqKey="Okada M" first="Masaji" last="Okada">Masaji Okada</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Takemoto, Yuji" sort="Takemoto, Yuji" uniqKey="Takemoto Y" first="Yuji" last="Takemoto">Yuji Takemoto</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Okuno, Yoshinobu" sort="Okuno, Yoshinobu" uniqKey="Okuno Y" first="Yoshinobu" last="Okuno">Yoshinobu Okuno</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Infectious Diseases, Osaka Prefectural Institute of Public Health</s1><s2>3-69 Nakamichi 1-chome Higashinari-ku, Osaka 537-0025</s2><s3>JPN</s3><sZ>3 aut.</sZ><sZ>18 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>3-69 Nakamichi 1-chome Higashinari-ku, Osaka 537-0025</wicri:noRegion></affiliation></author><author><name sortKey="Hashimoto, Satomi" sort="Hashimoto, Satomi" uniqKey="Hashimoto S" first="Satomi" last="Hashimoto">Satomi Hashimoto</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Yoshida, Shigeto" sort="Yoshida, Shigeto" uniqKey="Yoshida S" first="Shigeto" last="Yoshida">Shigeto Yoshida</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Infection and Immunity, Jichi Medical School, 3311-1 Yakushiji</s1><s2>Minamikawachi-machi, Tochigi 329-0498</s2><s3>JPN</s3><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Minamikawachi-machi, Tochigi 329-0498</wicri:noRegion></affiliation></author><author><name sortKey="Fukunaga, Yukari" sort="Fukunaga, Yukari" uniqKey="Fukunaga Y" first="Yukari" last="Fukunaga">Yukari Fukunaga</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Tanaka, Takao" sort="Tanaka, Takao" uniqKey="Tanaka T" first="Takao" last="Tanaka">Takao Tanaka</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Kita, Yoko" sort="Kita, Yoko" uniqKey="Kita Y" first="Yoko" last="Kita">Yoko Kita</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Kuwayama, Sachiko" sort="Kuwayama, Sachiko" uniqKey="Kuwayama S" first="Sachiko" last="Kuwayama">Sachiko Kuwayama</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Muraki, Yumiko" sort="Muraki, Yumiko" uniqKey="Muraki Y" first="Yumiko" last="Muraki">Yumiko Muraki</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Kanamaru, Noriko" sort="Kanamaru, Noriko" uniqKey="Kanamaru N" first="Noriko" last="Kanamaru">Noriko Kanamaru</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Takai, Hiroko" sort="Takai, Hiroko" uniqKey="Takai H" first="Hiroko" last="Takai">Hiroko Takai</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Okada, Chika" sort="Okada, Chika" uniqKey="Okada C" first="Chika" last="Okada">Chika Okada</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Sakaguchi, Yayoi" sort="Sakaguchi, Yayoi" uniqKey="Sakaguchi Y" first="Yayoi" last="Sakaguchi">Yayoi Sakaguchi</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Furukawa, Izumi" sort="Furukawa, Izumi" uniqKey="Furukawa I" first="Izumi" last="Furukawa">Izumi Furukawa</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Yamada, Kyoko" sort="Yamada, Kyoko" uniqKey="Yamada K" first="Kyoko" last="Yamada">Kyoko Yamada</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author><author><name sortKey="Matsumoto, Makoto" sort="Matsumoto, Makoto" uniqKey="Matsumoto M" first="Makoto" last="Matsumoto">Makoto Matsumoto</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Microbiological Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10, Kagasuno</s1><s2>Kawauchi-cho, Tokushima 771-019</s2><s3>JPN</s3><sZ>17 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Kawauchi-cho, Tokushima 771-019</wicri:noRegion></affiliation></author><author><name sortKey="Kase, Tetsuo" sort="Kase, Tetsuo" uniqKey="Kase T" first="Tetsuo" last="Kase">Tetsuo Kase</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Infectious Diseases, Osaka Prefectural Institute of Public Health</s1><s2>3-69 Nakamichi 1-chome Higashinari-ku, Osaka 537-0025</s2><s3>JPN</s3><sZ>3 aut.</sZ><sZ>18 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>3-69 Nakamichi 1-chome Higashinari-ku, Osaka 537-0025</wicri:noRegion></affiliation></author><author><name sortKey="Demello, Daphne E" sort="Demello, Daphne E" uniqKey="Demello D" first="Daphne E." last="Demello">Daphne E. Demello</name><affiliation wicri:level="2"><inist:fA14 i1="05"><s1>Department of Pathology Cardinal Glennon Children's Hospital, St. Louis University Health Science Center, 1465 South Grand Blvd</s1><s2>St. Louis, MO 63104</s2><s3>USA</s3><sZ>19 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Missouri (État)</region></placeName></affiliation></author><author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J. S. M." last="Peiris">J. S. M. Peiris</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Department of Microbiology, The University of Hong Kong, Pokfulam Road</s1><s3>HKG</s3><sZ>20 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Department of Microbiology, The University of Hong Kong, Pokfulam Road</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Pei Jer" sort="Chen, Pei Jer" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Hepatitis Research Center, National Taiwan University College of Medicine, Room 328, 3F, No. 1, Sec. 1, Ren-ai Rd</s1><s2>Jhongjheng District 100, Taipei</s2><s3>TWN</s3><sZ>21 aut.</sZ></inist:fA14><country>Taïwan</country><wicri:noRegion>Jhongjheng District 100, Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Tokyo Medical and Dental University, 1-5-45 Yushima</s1><s2>Bunkyo-ku, Tokyo 113-8549</s2><s3>JPN</s3><sZ>22 aut.</sZ><sZ>23 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Yoshinaka, Yoshiyuki" sort="Yoshinaka, Yoshiyuki" uniqKey="Yoshinaka Y" first="Yoshiyuki" last="Yoshinaka">Yoshiyuki Yoshinaka</name><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Tokyo Medical and Dental University, 1-5-45 Yushima</s1><s2>Bunkyo-ku, Tokyo 113-8549</s2><s3>JPN</s3><sZ>22 aut.</sZ><sZ>23 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Nomura, Tatsuji" sort="Nomura, Tatsuji" uniqKey="Nomura T" first="Tatsuji" last="Nomura">Tatsuji Nomura</name><affiliation wicri:level="1"><inist:fA14 i1="09"><s1>Central Institute for Experimental Animals, 1430 Nogawa, Miyamae</s1><s2>Kawasaki, Kanagawa 216-0001</s2><s3>JPN</s3><sZ>24 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Kawasaki, Kanagawa 216-0001</wicri:noRegion></affiliation></author><author><name sortKey="Ishida, Isao" sort="Ishida, Isao" uniqKey="Ishida I" first="Isao" last="Ishida">Isao Ishida</name><affiliation wicri:level="1"><inist:fA14 i1="10"><s1>Pharmaceutical Division, Kirin Brewery Co., 6-26-1 Jingumae</s1><s2>Shibuya, Tokyo 150-8011</s2><s3>JPN</s3><sZ>25 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name><affiliation wicri:level="1"><inist:fA14 i1="11"><s1>National Institute of Infectious Diseases, 1-23-1 Toyama</s1><s2>Shinjuku-ku, Tokyo 162-8640</s2><s3>JPN</s3><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name><affiliation wicri:level="1"><inist:fA14 i1="11"><s1>National Institute of Infectious Diseases, 1-23-1 Toyama</s1><s2>Shinjuku-ku, Tokyo 162-8640</s2><s3>JPN</s3><sZ>26 aut.</sZ><sZ>27 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Sakatani, Mitsunori" sort="Sakatani, Mitsunori" uniqKey="Sakatani M" first="Mitsunori" last="Sakatani">Mitsunori Sakatani</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Clinical Research Center, National Hospital Organization Kinki-Chuo Chest Medical Center, 1180 Nagasone</s1><s2>Sakai, Osaka 591-8555</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>12 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ><sZ>15 aut.</sZ><sZ>16 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sakai, Osaka 591-8555</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Vaccine</title><title level="j" type="abbreviated">Vaccine</title><idno type="ISSN">0264-410X</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Vaccine</title><title level="j" type="abbreviated">Vaccine</title><idno type="ISSN">0264-410X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Antigens, Viral (genetics)</term><term>Combined immune deficiency</term><term>Cytotoxicity</term><term>Female</term><term>Genetic vaccine</term><term>Human</term><term>Humans</term><term>In Vitro Techniques</term><term>Lymphocyte Activation</term><term>Lymphocyte Transfusion</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Inbred C57BL</term><term>Mice, Inbred NOD</term><term>Mice, Knockout</term><term>Mice, SCID</term><term>Mouse</term><term>Neutralization Tests</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Severe</term><term>Severe acute respiratory syndrome</term><term>Severe acute respiratory syndrome virus</term><term>T-Lymphocyte</term><term>T-Lymphocytes, Cytotoxic (immunology)</term><term>Transplantation, Heterologous</term><term>Vaccines, DNA (genetics)</term><term>Vaccines, DNA (pharmacology)</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (immunology)</term><term>Viral Vaccines (genetics)</term><term>Viral Vaccines (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation des lymphocytes</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Antigènes viraux (génétique)</term><term>Femelle</term><term>Humains</term><term>Lymphocytes T cytotoxiques (immunologie)</term><term>Protéines virales (génétique)</term><term>Protéines virales (immunologie)</term><term>Souris</term><term>Souris SCID</term><term>Souris de lignée BALB C</term><term>Souris de lignée C57BL</term><term>Souris de lignée NOD</term><term>Souris knockout</term><term>Techniques in vitro</term><term>Tests de neutralisation</term><term>Transfusion de lymphocytes</term><term>Transplantation hétérologue</term><term>Vaccins antiviraux (génétique)</term><term>Vaccins antiviraux (isolement et purification)</term><term>Vaccins à ADN (génétique)</term><term>Vaccins à ADN (pharmacologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Viral</term><term>Vaccines, DNA</term><term>Viral Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes viraux</term><term>Protéines virales</term><term>Vaccins antiviraux</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lymphocytes T cytotoxiques</term><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>T-Lymphocytes, Cytotoxic</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Humans</term><term>In Vitro Techniques</term><term>Lymphocyte Activation</term><term>Lymphocyte Transfusion</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Inbred C57BL</term><term>Mice, Inbred NOD</term><term>Mice, Knockout</term><term>Mice, SCID</term><term>Neutralization Tests</term><term>Transplantation, Heterologous</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Activation des lymphocytes</term><term>Animaux</term><term>Femelle</term><term>Humains</term><term>Souris</term><term>Souris SCID</term><term>Souris de lignée BALB C</term><term>Souris de lignée C57BL</term><term>Souris de lignée NOD</term><term>Souris knockout</term><term>Techniques in vitro</term><term>Tests de neutralisation</term><term>Transfusion de lymphocytes</term><term>Transplantation hétérologue</term><term>Virus syndrome respiratoire aigu sévère</term><term>Souris</term><term>Homme</term><term>Vaccin génétique</term><term>Grave</term><term>Lymphocyte T</term><term>Cytotoxicité</term><term>Immunodéficit mixte</term><term>Syndrome respiratoire aigu sévère</term><term>Mutation severe combined immunodeficiency (scid)</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have investigated to develop novel vaccines against SARS CoV using cDNA constructs encoding the structural antigen; spike protein (S), membrane protein (M), envelope protein (E), or nucleocapsid (N) protein, derived from SARS CoV. Mice vaccinated with SARS-N or -M DNA using pcDNA 3.1(+) plasmid vector showed T cell immune responses (CTL induction and proliferation) against N or M protein, respectively. CTL responses were also detected to SARS DNA-transfected type II alveolar epithelial cells (T7 cell clone), which are thought to be initial target cells for SARS virus infection in human. To determine whether these DNA vaccines could induce T cell immune responses in humans as well as in mice, SCID-PBL/hu mice was immunized with these DNA vaccines. As expected, virus-specific CTL responses and T cell proliferation were induced from human T cells. SARS-N and SARS-M DNA vaccines and SCID-PBL/hu mouse model will be important in the development of protective vaccines.</div></front></TEI><affiliations><list><country><li>Hong Kong</li><li>Japon</li><li>Taïwan</li><li>États-Unis</li></country><region><li>Missouri (État)</li><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><noRegion><name sortKey="Okada, Masaji" sort="Okada, Masaji" uniqKey="Okada M" first="Masaji" last="Okada">Masaji Okada</name></noRegion><name sortKey="Fukunaga, Yukari" sort="Fukunaga, Yukari" uniqKey="Fukunaga Y" first="Yukari" last="Fukunaga">Yukari Fukunaga</name><name sortKey="Furukawa, Izumi" sort="Furukawa, Izumi" uniqKey="Furukawa I" first="Izumi" last="Furukawa">Izumi Furukawa</name><name sortKey="Hashimoto, Satomi" sort="Hashimoto, Satomi" uniqKey="Hashimoto S" first="Satomi" last="Hashimoto">Satomi Hashimoto</name><name sortKey="Ishida, Isao" sort="Ishida, Isao" uniqKey="Ishida I" first="Isao" last="Ishida">Isao Ishida</name><name sortKey="Kanamaru, Noriko" sort="Kanamaru, Noriko" uniqKey="Kanamaru N" first="Noriko" last="Kanamaru">Noriko Kanamaru</name><name sortKey="Kase, Tetsuo" sort="Kase, Tetsuo" uniqKey="Kase T" first="Tetsuo" last="Kase">Tetsuo Kase</name><name sortKey="Kita, Yoko" sort="Kita, Yoko" uniqKey="Kita Y" first="Yoko" last="Kita">Yoko Kita</name><name sortKey="Kuwayama, Sachiko" sort="Kuwayama, Sachiko" uniqKey="Kuwayama S" first="Sachiko" last="Kuwayama">Sachiko Kuwayama</name><name sortKey="Matsumoto, Makoto" sort="Matsumoto, Makoto" uniqKey="Matsumoto M" first="Makoto" last="Matsumoto">Makoto Matsumoto</name><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name><name sortKey="Muraki, Yumiko" sort="Muraki, Yumiko" uniqKey="Muraki Y" first="Yumiko" last="Muraki">Yumiko Muraki</name><name sortKey="Nomura, Tatsuji" sort="Nomura, Tatsuji" uniqKey="Nomura T" first="Tatsuji" last="Nomura">Tatsuji Nomura</name><name sortKey="Okada, Chika" sort="Okada, Chika" uniqKey="Okada C" first="Chika" last="Okada">Chika Okada</name><name sortKey="Okuno, Yoshinobu" sort="Okuno, Yoshinobu" uniqKey="Okuno Y" first="Yoshinobu" last="Okuno">Yoshinobu Okuno</name><name sortKey="Sakaguchi, Yayoi" sort="Sakaguchi, Yayoi" uniqKey="Sakaguchi Y" first="Yayoi" last="Sakaguchi">Yayoi Sakaguchi</name><name sortKey="Sakatani, Mitsunori" sort="Sakatani, Mitsunori" uniqKey="Sakatani M" first="Mitsunori" last="Sakatani">Mitsunori Sakatani</name><name sortKey="Takai, Hiroko" sort="Takai, Hiroko" uniqKey="Takai H" first="Hiroko" last="Takai">Hiroko Takai</name><name sortKey="Takemoto, Yuji" sort="Takemoto, Yuji" uniqKey="Takemoto Y" first="Yuji" last="Takemoto">Yuji Takemoto</name><name sortKey="Tanaka, Takao" sort="Tanaka, Takao" uniqKey="Tanaka T" first="Takao" last="Tanaka">Takao Tanaka</name><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name><name sortKey="Yamada, Kyoko" sort="Yamada, Kyoko" uniqKey="Yamada K" first="Kyoko" last="Yamada">Kyoko Yamada</name><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><name sortKey="Yoshida, Shigeto" sort="Yoshida, Shigeto" uniqKey="Yoshida S" first="Shigeto" last="Yoshida">Shigeto Yoshida</name><name sortKey="Yoshinaka, Yoshiyuki" sort="Yoshinaka, Yoshiyuki" uniqKey="Yoshinaka Y" first="Yoshiyuki" last="Yoshinaka">Yoshiyuki Yoshinaka</name></country><country name="États-Unis"><region name="Missouri (État)"><name sortKey="Demello, Daphne E" sort="Demello, Daphne E" uniqKey="Demello D" first="Daphne E." last="Demello">Daphne E. Demello</name></region></country><country name="Hong Kong"><noRegion><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J. S. M." last="Peiris">J. S. M. Peiris</name></noRegion></country><country name="Taïwan"><noRegion><name sortKey="Chen, Pei Jer" sort="Chen, Pei Jer" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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